You will work as an unexpected emergency room doctor in Topeka when a mom brings in her 8 yr old son because of severe, bloody diarrhea. When ever questioned, the boy declared that it hurts when he goes to the bathroom great mother said he had vomited in the previous 2 days. When mentioned their prior movements, the mother and son both mentioned an outdoor barbeque that had occurred 6 times earlier. The boy lamented that his mother built him eat a “pink burger that has not been thoroughly cooked.


A physical study of the young man revealed absolutely nothing out of the ordinary except dehydration in the diarrhea. Lab tests showed that he had 13, 000 white blood cells/microliter of blood vessels, and a methylene blue stain of his fecal material showed considerable neutrophils. His stool specimen was finished on MacConkey agar and lots of colonies on the plate converted bright red after twenty four hours. Based on symptoms and the laboratory results, having been treated with trimethoprim-sulfamethoxadole and intravenous substance therapy.

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He improved quickly and was released after 24 hours.

1 . What micro organism is the most probably cause of the infection? Describe the clues you used to determine this away. (3 pts)The microbe probably causing this infection may be the parasite E. Coli. The uncooked burger can contain the E. Coli bacteria that comes from cows intestines. We currently have E. Coli present in our intestines although large amounts in the bacteria may be toxic. According to the Mayo Clinic staff, symptoms of large amounts of E. Coli in the intestinal tract include weakling diarrhea, stomach cramping, discomfort, or pain, and nausea and vomiting. The eight year old little boy showed all these symptoms.

Sources: -Golder, Ellie. Nurse Practitioner. Personal Interview. November 6, 2012. – “E. Coli.  Mayo Center. July twenty eight, 2011. The fall of 6, 2012.

2 . Discover and identify one of the important virulence elements produced by the causative agent. (Hint: there exists more than one proper answer here! ) How does this intensité factor contribute to the ability of the organism to cause disease? (3 pts)One important intensité factor produced by E. Coli is harmful toxins. Toxins happen to be proteins secreted by the bacterias that are poisonous to the sponsor. In this case the toxins that are made by At the. Coli, damage the lining with the small intestinal tract and that is what can cause the weakling diarrhea. Resources:

-Leboffie, Michael J. Microbiology. Burton At the. Pierce. Morton. Englewood, Co. 2012

3. Based on everything you know about the selective and differential capabilities of MacConkey agar, would it be likely to obtain similar results from a stool sample of any health individual? Why or perhaps why not? (3 pts)The MacConkey agar is capable of developing E. Coli because it prevents the growth of Gram-positive bacterias and Electronic. Coli is actually a gram-negative bacteria. If we had taken a stool sample from a wholesome patient the selective multimedia would be similar because Elizabeth. Coli always grows on the MacConkey agar agar and Electronic. Coli is always present in the human intestines. Though the differential mass media would not end up being similar as a result of amounts of E. Coli inside the intestines and also whether it is infectious or certainly not. Different kinds of At the. Coli have the ability or inability to levain lactose which in turn changes the colors of the dyes after the incubation period.


-Leboffie, Michael L. Microbiology. Burton E. Touch. Morton. Englewood, Co. 2012

4. Just how is this affected person spread? How could infection be prevented? (3 pts)

In line with the Mayo Center Staff, Electronic. Coli is most easily distributed through the ingestion of meals. In this case, the land beef the little boy used was polluted with Electronic. Coli. The moment cattle is definitely slaughtered and processed a number of the E. Coli from their intestinal tract can ruin the various meats. Other meals that can be infected with Elizabeth. Coli is usually unpasteurized milk, fruits and also other produce, and restaurant meals when machines and at home cooks do not clean their hands thoroughly after using the restroom. Sources: – “E. Coli.  Mayonaise Clinic. September 28, 2011. November six, 2012.

a few. Was antibiotic therapy the right choice for this patient? For what reason or why not?

(3 pts)

Antibiotic therapy likely wasn’t the best option for the limited boy. The moment discussing the truth with nurse practitioner, Kim Golder, she advised that the best treatment option for an E. Coli infection should be to drink a good amount of fluids and so the patient does not get dried out. She also recommended to get lots of foundation rest and wait for the disease to diminish.

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